F4H5 Alleviates Increased Outflow Resistance Induced by Emulsified Silicone Oil In Vitro.
Muuss Marcel, Karaivanova Margarita, Skrzypczyk Lea, Wohlfart Sabrina, Herth Jonathan, Uhl Philipp, Auffarth Gerd Uwe, Hammer Maximilian
AI Summary
Emulsified silicone oil increases outflow resistance, raising IOP. This study found F4H5 effectively reverses this IOP increase in human and porcine eye models, suggesting a potential treatment for silicone oil-induced glaucoma.
Abstract
Purpose
The purpose of this study was to investigate the effect of emulsified silicone oil on trabecular outflow resistance using an in vitro perfusion model of human and porcine anterior segments. F4H5, an approved washout for emulsified silicone oil in the posterior segment, was evaluated as a potential rescue treatment.
Methods
Seventeen porcine and 15 human anterior segments were dissected and clamped into an in vitro perfusion model. The intraocular pressure (IOP) was recorded continuously and evaluated before and after the injection of emulsified silicone oil, followed by treatment with F4H5 in the intervention group. Control eyes received injections of balanced salt solution (BSS).
Results
In human eyes, the injection of emulsified silicone oil significantly increased IOP from 7.8 ± 1.3 millimeters of mercury (mm Hg) to 15.8 ± 3.5 mm Hg (increase of 8.0 ± 2.3 mm Hg, P = 0.02, mean time to stabilized higher pressure = 8.6 hours). Subsequent treatment with F4H5 reverted the IOP back to near baseline values (10.8 ± 2.2 mm Hg, P = 0.06 compared to baseline, mean time to stabilized lower pressure = 6.9 hours). Porcine eyes demonstrated comparable trends, with a significant IOP increase from 14.5 ± 1.2 mm Hg to 17.7 ± 1.6 mm Hg after silicone oil injection (increase of 3.2 ± 0.9 mm Hg, P = 0.01) and a subsequent significant drop in IOP to 12.4 ± 1.0 mm Hg after the injection of F4H5 (decrease of 5.3 ± 1.1 mm Hg, P < 0.01).
Conclusions
Emulsified silicone oil leads to blockage of the outflow pathways and an increased IOP in an in vitro human and porcine anterior segment perfusion model. With F4H5, a clinically relevant reversal of this increased outflow resistance was achieved and should be further evaluated.
MeSH Terms
Shields Classification
Key Concepts4
In an in vitro perfusion model of human anterior segments (n=15), the injection of emulsified silicone oil significantly increased intraocular pressure (IOP) from 7.8 ± 1.3 mm Hg to 15.8 ± 3.5 mm Hg (increase of 8.0 ± 2.3 mm Hg, P = 0.02) with a mean time to stabilized higher pressure of 8.6 hours.
Following the significant IOP increase induced by emulsified silicone oil in an in vitro perfusion model of human anterior segments (n=15), subsequent treatment with F4H5 reverted the IOP back to near baseline values (10.8 ± 2.2 mm Hg, P = 0.06 compared to baseline) with a mean time to stabilized lower pressure of 6.9 hours.
In an in vitro perfusion model of porcine anterior segments (n=17), emulsified silicone oil injection caused a significant intraocular pressure (IOP) increase from 14.5 ± 1.2 mm Hg to 17.7 ± 1.6 mm Hg (increase of 3.2 ± 0.9 mm Hg, P = 0.01).
In an in vitro perfusion model of porcine anterior segments (n=17), after the IOP increase induced by emulsified silicone oil, injection of F4H5 resulted in a subsequent significant drop in IOP to 12.4 ± 1.0 mm Hg (decrease of 5.3 ± 1.1 mm Hg, P < 0.01).
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