Investigation of corneal endothelial changes post selective laser trabeculoplasty.
Leahy Kate E, Madigan Michele C, Sarris Maria, Watson Stephanie L, McCluskey Peter, White Andrew Jr
AI Summary
SLT causes transient corneal endothelial tight junction changes (reduced ZO-1), possibly via free radicals, explaining observed post-procedure corneal edema.
Abstract
Importance
Transient corneal endothelial changes are routinely noted on slit-lamp examination immediately following selective laser trabeculoplasty (SLT).
Background
To determine the mechanism of transient corneal endothelial changes observed following SLT.
Design
University laboratory-based observational study.
Samples: Ten corneas from six human cadaveric donors.
Methods
Corneas were treated with SLT, direct laser or peroxide, or used as controls. Haematoxylin and eosin staining and immunolabelling for zonula occludens-1 (ZO-1) and beta-catenin were performed.
Main outcome measures
Histological appearance; ZO-1 and beta-catenin immunostaining.
Results
There were no differences in histological features between SLT-treated and control corneas. Corneas treated with SLT or peroxide showed reduced and less regular ZO-1 immunofluorescence along cell membranes compared with ZO-1 expression in controls. These changes were generalized across the endothelium. There was no effect on the ZO-1 immunostaining after direct laser. There was no difference in beta-catenin immunostaining patterns between control, SLT and peroxide-treated corneas.
Conclusions and relevance: Altered ZO-1 immunostaining may represent disassembly of tight junctions between corneal endothelial cells. The similarity of our findings between SLT-treated and peroxide-treated corneas suggests that both conditions trigger changes at the level of endothelial tight junctions, perhaps triggered by liberation of free radicals as previously proposed.
MeSH Terms
Shields Classification
Key Concepts5
There were no differences in histological features between corneas treated with selective laser trabeculoplasty (SLT) and control corneas in a university laboratory-based observational study using 10 corneas from six human cadaveric donors.
Corneas treated with selective laser trabeculoplasty (SLT) or peroxide showed reduced and less regular zonula occludens-1 (ZO-1) immunofluorescence along cell membranes compared with ZO-1 expression in controls, generalized across the endothelium, in a university laboratory-based observational study using 10 corneas from six human cadaveric donors.
There was no effect on the zonula occludens-1 (ZO-1) immunostaining after direct laser treatment in a university laboratory-based observational study using 10 corneas from six human cadaveric donors.
There was no difference in beta-catenin immunostaining patterns between control, selective laser trabeculoplasty (SLT) treated, and peroxide-treated corneas in a university laboratory-based observational study using 10 corneas from six human cadaveric donors.
Altered zonula occludens-1 (ZO-1) immunostaining following selective laser trabeculoplasty (SLT) may represent disassembly of tight junctions between corneal endothelial cells, suggesting that SLT triggers changes at the level of endothelial tight junctions, possibly due to the liberation of free radicals, based on findings from a university laboratory-based observational study using 10 corneas from six human cadaveric donors.
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