Sodium Hyaluronate-Induced Ocular Hypertension in Rats Damages the Direction-Selective Circuit and Inner/Outer Retinal Plexiform Layers.
Noailles Agustina, Kutsyr Oksana, Mayordomo-Febrer Aloma, Lax Pedro, López-Murcia María, Sanz-González Silvia M, Pinazo-Durán María Dolores, Cuenca Nicolás
AI Summary
This rat study found chronic ocular hypertension damages specific retinal cells and layers, particularly those involved in motion perception, which may explain visual abnormalities in glaucoma patients.
Abstract
Purpose
To assess the changes in retinal morphology in a rat model of chronic glaucoma induced by ocular hypertension.
Methods
Intraocular pressure (IOP) was surgically increased through weekly injections of sodium hyaluronate (HYA) in the anterior eye chamber of the left eye of male Wistar rats, whereas the right eyes were sham operated (salt solution). During the 10-week experimental period, IOP was measured weekly with a rebound tonometer. Retinal cryosections were prepared for histological/immunohistochemical analysis and morphometry.
Results
IOP was higher in HYA-treated eyes than in sham-operated eyes along the 10-week period, which was significant from the fourth to the nineth week. Ocular hypertension in HYA-treated eyes was associated with morphologic and morphometric changes in bipolar cells, ON-OFF direction-selective ganglion cells, ON/OFF starburst amacrine cells, and inner plexiform layer sublamina.
Conclusions
Serial HYA treatment in the rat anterior eye chamber results in mild-to-moderate elevated and sustained IOP and ganglion cell death, which mimics most human open-angle glaucoma hallmarks. The reduced number of direction-selective ganglion cells and starburst amacrine cells accompanied by a deteriorated ON/OFF plexus in this glaucoma model could lend insight to the abnormalities in motion perception observed in patients with glaucoma.
MeSH Terms
Shields Classification
Key Concepts4
Intraocular pressure (IOP) was significantly higher in male Wistar rat eyes treated with weekly injections of sodium hyaluronate (HYA) in the anterior eye chamber compared to sham-operated eyes (salt solution) from the fourth to the ninth week of a 10-week experimental period.
Ocular hypertension induced by serial sodium hyaluronate (HYA) treatment in male Wistar rats was associated with morphological and morphometric changes in bipolar cells, ON-OFF direction-selective ganglion cells, ON/OFF starburst amacrine cells, and the inner plexiform layer sublamina.
Serial sodium hyaluronate (HYA) treatment in the anterior eye chamber of rats results in mild-to-moderate elevated and sustained intraocular pressure (IOP) and ganglion cell death, mimicking most human open-angle glaucoma hallmarks.
The reduced number of direction-selective ganglion cells and starburst amacrine cells accompanied by a deteriorated ON/OFF plexus in a rat model of glaucoma induced by serial sodium hyaluronate (HYA) treatment could provide insight into abnormalities in motion perception observed in patients with glaucoma.
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