Cytokine Levels in the Aqueous Humor Are Associated With Corneal Thickness in Eyes With Bullous Keratopathy.
Suzuki Natsume, Yamaguchi Takefumi, Shibata Shinsuke, Nagai Toshihiro, Noma Hisashi, Tsubota Kazuo, Shimazaki Jun
AI Summary
This study found that corneal edema severity in bullous keratopathy correlates with specific inflammatory cytokines (IL-13, sICAM-1) in the aqueous humor, suggesting a role for inflammation in its pathogenesis.
Abstract
Purpose
We sought to investigate the association between the severity of bullous keratopathy and proinflammatory cytokine levels in the aqueous humor (AqH).
Design
Cross-sectional study.
Methods
This study included a total of 95 eyes: 62 with bullous keratopathy and 33 that underwent cataract surgery. Central corneal thickness (CCT) and central corneal volume within 4 and 6 mm (CCV 4mm and CCV 6mm , respectively) were determined using anterior segment optical coherence tomography. A total of 95 AqH samples were collected at the beginning of surgery. The levels of cytokines (interleukins [ILs]-1α, -1β, -4, -6, -8, -10, -12p70, -13, -17A, interferon [IFN]-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin, P-selectin, and soluble intercellular adhesion molecule-1 [sICAM-1]) in the AqH were measured using multiplex beads immunoassay. We evaluated the correlation among AqH cytokine levels, CCT, CCV 4mm , and CCV 6mm in eyes with bullous keratopathy.
Results
The levels of protein, ILs-4, -6, -8, -10, -12p70, and -17A, MCP-1, IFN-γ, E-selectin, P-selectin, and sICAM-1 were significantly higher in eyes with bullous keratopathy compared with those of the normal control subjects (all P < .0025). CCT was significantly correlated with the levels of IL-13 (r = 0.551, P = .0009) and sICAM-1 (r = 0.448, P = .0005). CCV 4mm was significantly correlated with the levels of IL-13 (r = 0.514, P = .0022) and sICAM-1 (r = 0.404, P = .0019). CCV 6mm was significantly correlated with the level of sICAM-1 (r = 0.459, P = .0003).
Conclusion
The severity of corneal edema in eyes with bullous keratopathy was associated with the levels of specific cytokines in the AqH.
MeSH Terms
Shields Classification
Key Concepts4
In a cross-sectional study of 95 eyes (62 with bullous keratopathy and 33 controls undergoing cataract surgery), the levels of protein, interleukins (ILs)-4, -6, -8, -10, -12p70, and -17A, monocyte chemotactic protein (MCP)-1, interferon (IFN)-γ, E-selectin, P-selectin, and soluble intercellular adhesion molecule-1 (sICAM-1) in the aqueous humor were significantly higher in eyes with bullous keratopathy compared with those of the normal control subjects (all P < .0025).
In a cross-sectional study of 62 eyes with bullous keratopathy, central corneal thickness (CCT) was significantly correlated with the levels of IL-13 (r = 0.551, P = .0009) and sICAM-1 (r = 0.448, P = .0005) in the aqueous humor.
In a cross-sectional study of 62 eyes with bullous keratopathy, central corneal volume within 4mm (CCV 4mm) was significantly correlated with the levels of IL-13 (r = 0.514, P = .0022) and sICAM-1 (r = 0.404, P = .0019) in the aqueous humor.
In a cross-sectional study of 62 eyes with bullous keratopathy, central corneal volume within 6mm (CCV 6mm) was significantly correlated with the level of sICAM-1 (r = 0.459, P = .0003) in the aqueous humor.
Related Articles5
Evaluation of Anterior Scleral Thickness and Angle Parameters in Eyes with Pseudoexfoliation Syndrome and Glaucoma.
Cross-Sectional StudyEvaluation of Anterior Segment Parameters in Pseudoexfoliation Disease Using Anterior Segment Optical Coherence Tomography.
Cross-Sectional StudyUltrasound biomicroscopy of angle closure glaucoma with pseudoexfoliation syndrome.
Case ReportQuadrantwise Comparison of Lens-Iris Distance in Patients With Pseudoexfoliation Syndrome and Age-matched Controls.
Cross-Sectional StudyIn vivo biometric evaluation of Schlemm's canal with spectral-domain optical coherence tomography in pseuduexfoliation glaucoma.
Observational StudyIs this article assigned to the wrong chapter(s)? Let us know.