Time-Dependent Effects of Reduced Cerebrospinal Fluid Pressure on Optic Nerve Retrograde Axonal Transport.
Zhang Zheng, Wu Shen, Liu Kegao, Zhang Jingxue, Liu Qian, Li Lei, Wang Ningli
AI Summary
This rat study found reduced cerebrospinal fluid pressure impairs optic nerve axonal transport after 3 hours, worsening over time. This suggests CSFP reduction contributes to glaucoma's nerve damage.
Abstract
Purpose
To study the time-dependent effects of reduced cerebrospinal fluid pressure (CSFP) on axonal transport in the rat optic nerve.
Methods
Seventy-two adult Sprague Dawley rats were used for this study. Fluoro-Gold was injected into the superior colliculi to study axonal transport. CSFP was reduced to 1.5 to 2.9 mm Hg by continuous aspiration of cerebrospinal fluid. In the sham control group (n = 18), a trocar was implanted in the cisterna magna, but cerebrospinal fluid was not released. CSFP and intraocular pressure (IOP) were continually monitored. CSFP was reduced for 1 hour (low-CSFP-1h study group; n = 18), 3 hours (low-CSFP-3h study group; n = 18), or 6 hours (low-CSFP-6h study group; n = 18) before the animals were euthanized. Confocal microscopy was used to compare axonal transport in different quadrants of the retina between control and low-CSFP eyes.
Results
Changes in axonal transport were observed only after 3 hours of CSFP reduction and not in the low-CSFP-1h study group. These changes occurred in a time-dependent manner, with 6 hours of CSFP reduction producing the longest lasting and most severe reduction in fluorescence.
Conclusions
The time-dependent changes observed in axonal transport in the optic nerve provide further evidence regarding the pathogenesis of axonal damage caused by reduced CSFP.
MeSH Terms
Shields Classification
Key Concepts3
Changes in axonal transport in the rat optic nerve were observed only after 3 hours of reduced cerebrospinal fluid pressure (CSFP) (1.5 to 2.9 mm Hg) and not in the low-CSFP-1h study group.
Reduced cerebrospinal fluid pressure (CSFP) (1.5 to 2.9 mm Hg) produced time-dependent changes in axonal transport in the rat optic nerve, with 6 hours of CSFP reduction resulting in the longest lasting and most severe reduction in fluorescence.
The time-dependent changes observed in axonal transport in the rat optic nerve due to reduced cerebrospinal fluid pressure (CSFP) provide further evidence regarding the pathogenesis of axonal damage caused by reduced CSFP.
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