Retinal Ganglion Cell Content Underlying Standard Automated Perimetry Size I to V Visual Sensitivities in the Non-Human Primate Experimental Glaucoma Model.
Venkata Srinivasan Varsha, Carter-Dawson Louvenia, Patel Nimesh B
AI Summary
This study in primates linked visual field sensitivities (sizes I-V) to retinal ganglion cell counts. It found a structure-function model that estimates RGC content from standard perimetry, improving glaucoma monitoring.
Abstract
Purpose
To determine the relationship between visual sensitivities from white-on-white Goldmann size I to V stimuli and the underlying retinal ganglion cell (RGC) content in the non-human primate (NHP) experimental glaucoma model.
Methods
Normative data were collected from 13 NHPs. Unilateral experimental glaucoma was induced in seven animals with the least variable fields who were monitored using optical coherence tomography and 30-2 full-threshold standard automated perimetry (SAP). At varying endpoints, animals were euthanized followed by perfusion fixation, and 1-mm retinal punches were obtained from 34 corresponding SAP locations. RGCs were immunolabeled with an antibody against an RNA-binding protein (RBPMS) marker and imaged using confocal microscopy. RGC counts from each location were then related to visual sensitivities for each stimulus size, after accounting for ocular magnification.
Results
At the endpoint, the circumpapillary retinal nerve fiber layer thickness for experimental glaucoma eyes ranged from 47 to 113 µm. RGC density in control eyes was greatest for the 4.24° sample (18,024 ± 6869 cells/mm2) and decreased with eccentricity. Visual sensitivity at each tested location followed that predicted by spatial summation, with the critical area increasing with eccentricity (slope = 0.0036, R2 = 0.44). The relationship between RGC counts and visual sensitivity was described using a two-line fit, where the intercept of the first segment and hinge points were dependent on eccentricity.
Conclusions
In NHPs, SAP visual thresholds are related to the underlying RGCs. The resulting spatial summation based structure-function model can be used to estimate RGC content from any standard white-on-white stimulus size.
MeSH Terms
Shields Classification
Key Concepts6
In non-human primates with experimental glaucoma, the circumpapillary retinal nerve fiber layer thickness at the endpoint ranged from 47 to 113 µm.
In control eyes of non-human primates, RGC density was greatest for the 4.24° sample (18,024 ± 6869 cells/mm2) and decreased with eccentricity.
In non-human primates, visual sensitivity at each tested location followed that predicted by spatial summation, with the critical area increasing with eccentricity (slope = 0.0036, R2 = 0.44).
In non-human primates, the relationship between retinal ganglion cell (RGC) counts and visual sensitivity was described using a two-line fit, where the intercept of the first segment and hinge points were dependent on eccentricity.
In non-human primates, standard automated perimetry (SAP) visual thresholds are related to the underlying retinal ganglion cells (RGCs), and the resulting spatial summation based structure-function model can be used to estimate RGC content from any standard white-on-white stimulus size.
In a study of 13 non-human primates (NHPs), the relationship between visual sensitivities from white-on-white Goldmann size I to V stimuli and the underlying retinal ganglion cell (RGC) content was determined in an experimental glaucoma model.
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