Invest Ophthalmol Vis SciDecember 2024Consensus Statement

Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches.

Authors

Ted S Acott, Michael P Fautsch, Weiming Mao, C Ross Ethier, Alex S Huang, Mary J Kelley, Mini Aga, Sanjoy K Bhattacharya, Terete Borras, Diane Bovenkamp, Uttio Roy Chowdhury, Abbot F Clark, Mohammed I Dibas, Yiqin Du, Michael H Elliott, Jennifer A Faralli, Haiyan Gong, Samuel Herberg, Murray A Johnstone, Paul L Kaufman, Kate E Keller, Ruth A Kelly, David Krizaj, Markus H Kuehn, Hoi Lam Li, Raquel Lieberman, Shan C Lin, Yutao Liu, Fiona S McDonnell, Colleen M McDowell, Gillian J McLellan, Philip Mzyk, Kayarat Saidas Nair, Darryl R Overby, Donna M Peters, VijayKrishna Raghunathan, Ponugoti Vasantha Rao, Gavin W Roddy, Najam A Sharif, Myoung Sup Shim, Yang Sun, Benjamin R Thomson, Carol B Toris, Colin E Willoughby, Hao F Zhang, Thomas F Freddo, Rudolf Fuchshofer, Kamisha R Hill, Alireza Karimi, Krishnakumar Kizhatil, Casey C Kopcyznski, Paloma Liton, Gaurang Patel, Michael Peng, Padmanabhan P Pattabiraman, Ganesh Prasanna, Ester Reina-Torres, E Griffen Samples, John R Samples, Cynthia L Steel, Clemens A Strohmaier, Preeti Subramanian, Chenna Kesavulu Sugali, Batenburg-Sherwood Joseph van, Cydney Wong, Hannah Youngblood, Gulab S Zode, Elizabeth White, W Daniel Stamer

IOP & Medical TherapyDisease Progression

4 citations Open Access

Summary

These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges.

Abstract

Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.