Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches.
Acott Ted S, Fautsch Michael P, Mao Weiming, Ethier C Ross, Huang Alex S, Kelley Mary J, Aga Mini, Bhattacharya Sanjoy K, Borras Terete, Bovenkamp Diane
AI Summary
This paper details ex vivo perfusion methods for studying eye pressure regulation, providing standardized tools to better understand outflow function, crucial for glaucoma research and treatment.
Abstract
Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.
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Shields Classification
Key Concepts4
Intraocular pressure (IOP) elevation is the primary risk factor for progression of glaucomatous optic neuropathy.
Intraocular pressure (IOP) elevation is currently the main treatable factor for progression of glaucomatous optic neuropathy.
Ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function, which is responsible for IOP regulation.
Well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation are presented, including perfused whole globes, stationary anterior segment organ culture, perfused human anterior segment organ culture, perfused animal anterior segment organ culture, perfused human corneal rims, and perfused human anterior segment wedges.
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